Plasmid sequencing directly from bacterial colonies and cultures.

This is the protocol for direct plasmid sequencing from bacterial colonies and cultures, so you send us an aliquot of culture or a bacterial colony picked from an agar plate as a template.

You supply:

  • The template is a bacterial suspension. In a separate tube you also supply sequencing primer(s). 
  • Only if you wish to use our universal primers, you supply the template only.

We perform:

  • Plasmid amplification
  • Mixing of the amplified plasmid and a sequencing primer in optimal ratio
  • Sequencing reaction
  • Capillary electrophoresis of sequencing reaction products
  • Manual data analysis

Sample requirements - ColonySeq

Time to results:

  • Typically up to 2 working days from the moment we receive your samples.

What you can expect:

  • Length of read up to 1100 bp, data accuracy typically > 99 %
  • Universal primers provided free of charge
  • Results online in .ab1, fasta, and .phd.1 format
  • Automatic email message as soon as your results are ready to download
  • Permanent internal quality control, repeated runs for free
  • Storage of your samples for one week, of your results for two years
  • Experienced technical support


Please notice that count of primers you can use is limited (1 primer for every 8 samples).



Data analysis:

The evaluation of the results obtained by the ColonySeq protocol is performed manually by our specialists! Each sample file is checked before sending and re-analyzed as needed to get the most out of it for you. We recommend to your attention the articles in our Magazine, where we continuously address various aspects of this method, including the evaluation of the results.


Comparison of our sequencing protocols - download HERE.




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