BGH-Reverse

TAGAAGGCACAGTCGAGG

BGH (bovine growth hormone) terminator, reverse primer

CMV-F

CGCAAATGGGCGGTAGGCGTG

CMV promoter, forward primer

EGFP-C

GATCACATGGTCCTGCTG

3’ end of EGFP, forward primer

EGFP-N

CCGTCCAGCTCGACCAG

5’ end of EGFP, reverse primer

M13F

TGTAAAACGACGGCCAGT

In lacZ gene

M13R

CAGGAAACAGCTATGACC

In lacZ gene

pGEX-3

GGAGCTGCATGTGTCAGAG

3’ end of MCS in pGEX vectors, reverse primer

pGEX-5

CTGGCAAGCCACGTTTGG

3’ end of glutathione-S-transferase, forward primer

SP6

ATTTAGGTGACACTATAGAA

SP6 promoter, forward primer

T3

ATTAACCCTCACTAAAGGGA

T3 promoter, forward primer

T7 promoter

TAATACGACTCACTATAGGG

T7 promoter, forward primer

T7 terminator

GCTAGTTATTGCTCAGCGG

T7 terminator, reverse primer