Follow our Sample submission guidelines.

• Samples must be purified after first PCR and delivered exactly in required concentration. Too low a concentration could lead to a low PCR yield, high concentration to different yields or even inhibition of the reaction (high DNA concentration may be associated with a high concentration of inhibitors). Please provide samples in 0,2 mL strips or 96-well plates.
• It is required that samples are provided with tails (overhangs) as described, purified from artifacts such as excess primers or adaptors, enzymes etc.
• Total lenght of DNA fragments must be between 200 and 900 bp!

How to design primers

Append to 5’ end of your primers: 

• Forward: 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-[locus specific sequence]
• Reverse: 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-[locus specific sequence]

Step 1 in your lab

You PCR amplify the target regions and mix all amplicons from the same sample into one pool.

Step 2 in the SEQme lab

We perform a second PCR in which we add indexes and sequencing adapters to your amplicons. Samples processed in this way are then sequenced according to your requirements - the service is flexible in terms of output.