How tos and FAQs

Guidelines for sample preparation for DNA metabarcoding - Tailed-amplicon sequencing (Illumina only)

Follow our Sample submission guidelines.

  • Samples must be purified after first PCR and delivered exactly in required concentration. Too low a concentration could lead to a low PCR yield, high concentration to different yields or even inhibition of the reaction (high DNA concentration may be associated with a high concentration of inhibitors). Please provide samples in 0,2 mL strips or 96-well plates.
  • It is required that samples are provided with tails (overhangs) as described, purified from artifacts such as excess primers or adaptors, enzymes etc.
  • Total lenght of DNA fragments must be between 200 and 900 bp!

How to design primers

Append to 5’ end of your primers: 

  • Forward: 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-[locus specific sequence]
  • Reverse: 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-[locus specific sequence]

 

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