Sanger sequencing is the technique of choice if you need to determine the nucleotide sequence of individual DNA fragments, most often vector constructs or PCR amplicons. It is based on the separation of fluorescently labeled products of the sequencing reaction using capillary electrophoresis depending on their molecular weight.
We offer the following Sanger sequencing protocols:
- StandardSeq - This is the most common protocol in our Sanger sequencing portfolio of services. You are sending us a template that is ready for sequencing (purified).
- RapidSeq - The fastest protocol in our portfolio of Sanger sequencing services. RapidSeq protocol samples are analyzed preferentially in the sequencers and their results are evaluated automatically. You can expect first results in about 10 hours.
- ColonySeq - This is a protocol for direct plasmid sequencing from bacterial colonies and cultures, you send us an aliquot of culture or a bacterial colony picked from an agar plate as a template.
- CleanSeq - This is a protocol for sequencing of PCR products, which also includes enzymatic cleanup of templates. You send us a template that is not ready for sequencing and must be purified first.
- HairpinSeq - This is a special sequencing protocol for sequencing difficult templates. Bidirectional sequencing is included in this service. You send us a template that is ready for sequencing (purified).
Data analysis:
With the exception of the RapidSeq protocol, our specialists evaluate the results manually! Each result is checked before sending and re-analyzed as necessary to get the most out of it for you. We recommend that you pay attention to the articles in our Blog, where we continuously address various aspects of this method, including the evaluation of results.
Deliverables:
- Length of read up to 1100 bp, data accuracy typically > 99 %
- Universal primers provided free of charge
- Results online in .ab1, fasta, and .phd.1 format
- Automatic email message as soon as your results are ready to download
- Permanent internal quality control
- Storage of your samples for one week, of your results for two years
- Experienced technical support
Comparison of our sequencing protocols - download HERE.
StandardSeq
Standard single read sequencing service for plasmids or PCR fragments. The template must be mixed with the sequencing primer unless sequencing with a universal primer is ordered.
RapidSeq
Rapid single read sequencing service for plasmids or PCR fragments. The template must be mixed with the sequencing primer unless sequencing with a universal primer is ordered.
ColonySeq
Direct plasmid sequencing from bacterial colonies and cultures, you send us an aliquot of culture or a bacterial colony picked from an agar plate as a template.
CleanSeq
Sequencing of PCR products including their enzymatic cleanup. You send us a template that is not ready for sequencing and must be purified first.
HairpinSeq
Special sequencing protocol for hairpins, GC-rich and other problematic templates. The price includes sequencing from both directions. You send us a template that is ready for sequencing (purified).