How tos and FAQs

What target regions do you use for taxonomic analysis?

Various hypervariable gene regions or whole genes can be used for sequencing taxonomic analysis. Selection of primer combinations that we use for the amplification of these regions is continuously expanded. 

A list of available markers:

Long-read sequencing technologies (Oxford Nanopore)

  • 16S (27F; 1492R)
  • 18S_ITS1_ITS2_LSU (ITS1-F_KYO2, LR7)
  • COXI (LCO1490; HCO2198)
  • COXI (BcdF01; BcdR04)
  • COXI (COI_DGNS_F; COI_DGNS_R)

Short-read sequencing technologies (Illumina)

  • 16S_V3-V4 (Arch_349F; Arch_806R)
  • 16S_V3-V4 (357F; 805R)
  • 16S_V3-V5 (357F; 926R)
  • 16S_V4 (515F; 806R)
  • 16S_V4N (519F; 798R)
  • 16S_V4-V5 (515F; 926R)
  • 18S_1391-Br (1391F; Br-7R)
  • 18S_V4 (V4_Balzano_F; V4_Balzano_R)
  • 18S_V4-V5 (574*F; 952R)
  • 18S_V6-V8 (V6-V8_new_F; V6-V8_new_R)
  • 18S (NS31_F, AML2_R)
  • ITS1 (ITS1Fngs; ITS2R)
  • ITS1-2 (ITS1Fngs; ITS4R)
  • ITS2 (ITS3F; ITS4R)

If you do not find the target marker you want in the list, please contact us.

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