How tos and FAQs

How do I order Sanger sequencing or Fragment analysis?

All orders are placed through our web interface. To order analyzes, you must...

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General rules of sample preparation

When ordering sequencing or fragment analysis in tubes, preferably please use 0.2 ml strips to send samples, they are easy to work with.

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How do I ship samples?

We recommend you insert samples into a quick-closing plastic bag. Fragment analysis samples can be (not necessarily) wrapped up in aluminium foil to prevent light access.

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StandardSeq – Preparation of samples

Sample is a template mixed together with sequencing primer in a single test tube / well or template only if you wish to use some of our universal primers. Template is a PCR product or plasmid.

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RapidSeq - Preparation of samples

Please use the instructions for the StandardSeq protocol.

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ColonySeq – Preparation of samples

Sample is bacterial cells from agar plate colony, liquid media culture, or glycerol stock. This protocol is designed for mid- to high-copy number plasmids (> 10 copies per cell).

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CleanSeq – Preparation of samples

Sample is an unpurified PCR product (aliquot of the PCR reaction) or other suitable template, amenable to enzymatic cleanup. You must also supply sequencing primer(s) or select some of our universal primers.

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HairpinSeq – Preparation of samples

Sample is typically a plasmid with a cloned hairpin or GC-rich stretch in the region of interest or PCR product. Price of the HairpinSeq protocol includes bidirectional sequencing (forward and reverse).

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What is the difference between sequencing protocols you offer?

StandardSeq is the basic protocol, we sequence your template. CleanSeq is in fact StandardSeq with template cleanup performed beforehand. HairpinSeq is a special protocol ...

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How are RapidSeq protocol results evaluated?

The evaluation of results obtained by the RapidSeq protocol takes place without the intervention of human hands.

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Other questions?

Do not hesitate to contact us
We will be glad to help you.

Richard Nádvorník
+420 602 102 790
info@seqme.eu

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