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SEQme was established in 2012 to provide full solutions in the field of
DNA sequencing and Real-Time PCR.

We conduct sample analyses, organize courses and workshops
focused on these techniques and provide instrument services.

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How do I sequence large templates directly?

Here and there we do receive a request to sequence large templates (i.e. chromosomal DNA, BACs, cosmids) directly. We can do this but similarly as for standard samples where short pcr products or plasmids serve as templates also here the key prerequisite…

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Sequencing of short PCR products

Sanger sequencing is associated with certain technical limitations, and one of the most important is read length. Typically, even with a perfectly flawless design, not more than a roughly 1,100 bases can be expected. But what is the lower limit of how…

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