Clients of our sequencing laboratory often require the preparation of sequencing libraries using rRNA depletion (ribodepletion), ie including rRNA removal. As we sometimes find that the results of rRNA removal are unsatisfactory, we performed tests described below.
Illumina sequencing technology is commonly referred to as short-read technology. In practice this means that it typically produces reads of hundreds of bases in length. Its current technological maximum in terms of read length is a kit for the MiSeq system that allows reading up to 600 b (2x300 setting). In this article, we have taken a closer look at some of the problems associated with this type of sequencing.
Illumina sequencing technology is currently dominating the market. It offers a number of advantages over other technologies but unfortunately this does not mean that it is completely free of imperfections.
If I had a nickel for every time we answered the “How should I quantify my DNA or RNA for NGS library prep?” question, I wouldn’t need to be writing this article. I’d be on a boat in the Mediterranean. But since I’m not on a boat anywhere, I thought I could perhaps collect several good reasons why using Nanodrop is not really the best way of doing this.
